Background and Objectives: There are rare and contrary reports about the effects of microbial biofilms formation on the physical, chemical and biological properties of soil. There is no possibility of direct study of microbial biofilms in the soil due to limited scientific techniques; therefore, other similar materials are used as analogue for biofilm. Arabic gum is one of the analogues of biofilm, which is also discussed in some case studies. However, so far no report on its effect on different soil texture classes has been presented. Therefore, in order to evaluate the effects of Arabic gum on several soil characteristics of two different soil texture classes, the present study was designed and conducted. Materials and Methods: To do this study, two different soil types including clay loam and loam texture classes and different amounts of Arabic gum (0, 5 and 10 g per kg of soil) with three replications were investigated in a factorial experiment in a completely randomized design (CRD). Arabic gum was mixed with soil and after one-month incubation time, the disturbed and undisturbed samples were taken from the soils and different soil characteristics including bulk density (Db), soil moisture content (θ s), saturated hydraulic conductivity (Ks), saturated water content, soil aggregate stability (WAS), mean weight diameter of aggregate (MWD), mass fractal (Dm) of aggregates, soil acidity (pH), organic carbon (OC) content, cation exchange capacity (CEC) and microbial respiration (Rs) were measured. Results: The results showed that the effect of applied amounts of Arabic gum on θ s, soil Db, Ks and Rs in loam soil was lower than that of clay loam, whereas the application of 10 g Arabic gum per kg soil in clay loam soil caused an increase by approximately 12, 18 and 317 percent, respectively, in the volumetric and gravimetric θ s and the Ks; while these effects were not seen in loam soils. Usage of 10 g Arabic gum per kg soil also reduced the Db of clay loam soil up to 5%, while no changes were found in the Db of loam soils. Also, in treated soils, both applied amounts of Arabic gum (5 and 10 g per kg soil) showed positive changes in soil OC and the WAS, MWD and Dm of aggregates, being independent from soil type. According to the results, the amount of 5 g Arabic gum per kg soil can be recommended to improve the characteristics of the clay loam soils. Conclusion: The use of Arabic gum can improve the physical, chemical and biological properties of soil. In fine-textures soils with higher contents of clay, much less amount of it is needed, while in coarse-textured soils, more amount of this polymer are needed; in the other words, the effect of microbial biofilm formation is more pronounced in fine-textured soils.